Research Paper Volume 12, Issue 11 pp 10223—10234

Long non-coding RNA SNHG1 activates HOXA1 expression via sponging miR-193a-5p in breast cancer progression

Figure 2. SNHG1 promotes breast cancer cell migration, invasion and proliferation in vitro. (A) Q-RT-PCR analysis of SNHG1 mRNA levels in MDA-MB-231 cells transfected with NC, si-SNHG1-1 and si-SNHG1-2 siRNAs. **p<0.01 compared with NC control. (B, C) Migration (B) and invasion (C) of MDA-MB-231 cells transfected with NC, si-SNHG1-1 and si-SNHG1-2 siRNAs were examined by transwell assay. Representative images of the cells migrated or invaded to the lower chamber side (top panel). Cell migration and invasion capacities were shown as a percentage of NC control (bottom panel). **p<0.01 compared with NC control. (D) Representative images of wound healing assay of MDA-MB-231 cells transfected with NC, si-SNHG1-1 and si-SNHG1-2 siRNAs at indicated time (left panel). Wound repair rate was quantified in the histogram (right panel). **p<0.01 compared with NC control. (E, F) Proliferation of MDA-MB-231 cells transfected with NC, si-SNHG1-1 and si-SNHG1-2 siRNAs was examined by CCK-8 assay (E) and colony formation assay (F). (G) Apoptosis of MDA-MB-231 cells transfected with NC, si-SNHG1-1 and si-SNHG1-2 siRNAs was measured using flow cytometry. **p<0.01 compared with NC control. (H) Western blot analysis of cleaved Caspase-3 in MDA-MB-231 cells transfected with NC, si-SNHG1-1 and si-SNHG1-2 siRNAs. Actin was used as loading control.