Research Paper Volume 12, Issue 11 pp 10223—10234

Long non-coding RNA SNHG1 activates HOXA1 expression via sponging miR-193a-5p in breast cancer progression

Figure 4. SNHG1 acts as a sponge of miR-193a-5p to activate HOXA1 expression. (A) Predicated binding sites of miR-193a-5p on SNHG1 gene. (B) Q-RT-PCR analysis of miR-193a-5p levels in MDA-MB-231 cells transfected with NC, si-SNHG1-1 and si-SNHG1-2 siRNAs. **p<0.01 compared with NC control. (C) Q-RT-PCR analysis of SNHG1 levels in MDA-MB-231 cells transfected with miR-NC and miR-193a-5p mimics. **p<0.01 compared with miR-NC control. (D) Q-RT-PCR analysis of miR-193a-5p levels in collected breast cancer tissues (Tumor) and adjacent normal tissues (Normal). n=86, p<0.0001. (E) Correlation between miR-193a-5p levels and SNHG1 levels in breast cancer tissues. r=-0.7308, p<0.0001. (F) Wild-type (WT) and mutated (Mut) binding sites of miR-193a-5p on HOXA1 3’UTR region. (G) Effects of miR-193a-5p and si-SNHG1 on wild-type and mutant HOXA1 3’UTR reporter gene vectors in MDA-MB-231 cells. **p<0.01 compared with mock control. (H) Western blot assay of HOXA1 in MDA-MB-231 cells transfected with miR-193a-5p mimcs or si-SNHG1. Actin was used as loading control.