Research Paper Volume 12, Issue 11 pp 10223—10234

Long non-coding RNA SNHG1 activates HOXA1 expression via sponging miR-193a-5p in breast cancer progression

Figure 5. Ectopic expression of HOXA1 reversed the effect of SNHG1 silencing on MDA-MB-231 cells. (A, B) Migration (A) and invasion (B) of MDA-MB-231 cells transfected with NC, si-SNHG1 and si-SNHG1+HOXA1 were examined by transwell assay. Representative images of the cells migrated or invaded to the lower chamber side (top panel). Cell migration and invasion capacities were shown as a percentage of NC control (bottom panel). **p<0.01 compared with si-SNHG1 group. (C, D) Proliferation of MDA-MB-231 cells transfected with NC, si-SNHG1 and si-SNHG1+HOXA1 was examined by CCK-8 assay (C) and colony formation assay (D). (E) Apoptosis of MDA-MB-231 cells transfected with NC, si-SNHG1 and si-SNHG1+HOXA1 was measured using flow cytometry. **p<0.01 compared with si-SNHG1 group.