Figure 2. Telomere visualization and evaluation after Q-FISH in metaphase spreads of bovine oocytes. Telomeres were detected by Q-FISH on MII oocytes using a probe targeting telomeric repeats (red) and DNA stained with DAPI (blue). (A) Oocyte Metaphase II spread, scale bar: 5 μm. (B) Mean fluorescence intensity of telomeric spots in all experimental groups. (C) shows the results for the mean number of telomeric spots in the three experimental groups. (D) shows the results for the mean telomeric spot area expressed in Pixels (pxls) in the three experimental groups. (control= control without supplementation, CDM=vehicle control with DMSO supplementation, 1nMRap= 1nM Rapamycin supplementation).