Figure 5. Effects of miR-623 on PI3K/AKT and Wnt/β-catenin signaling pathways. (A–C) Protein expressions of AKT, p-AKT, mTOR, p-mTOR, Cyclin D1, S6K/P70 in MDA-MB-453 and MCF7 cells were detected by western blot assay. (D–F) Protein expression levels of wnt3, β-catenin, E-cad, Notch1, and HEY1 in MDA-MB-453 and MCF7 cells were detected by western blot assay. GAPDH was the internal control. Relative amounts of proteins normalized to GAPDH were shown. Experiments showing identical results were performed three times. P values were determined using Student’s t-tests. *P<0.05 compared with O-NC. #P<0.05 compared with siNC.