Figure 3. Effects of tomatidine on the viability of primary chondrocytes. (A) Chemical structure of tomatidine. (B, C) CCK-8 assay shows the viability of primary chondrocytes treated for 24 h with (B) 2.5, 5, 10 or 20 μM tomatidine alone or in combination with 10 ng/mlIL-1β (C). DMSO was used as control. As shown, treatments with 2.5, 5, or 10μM tomatidine or 10ng/mlIL-1β do not affect cell viability. Viability of primary chondrocytes is significantly affected by treatment with 20μM tomatidine in presence or absence of 10ng/mlIL-1β. The values are shown as means ± SD. *p < 0.05compared with the control group.