Research Paper Volume 12, Issue 13 pp 12799—12811

Tomatidine suppresses inflammation in primary articular chondrocytes and attenuates cartilage degradation in osteoarthritic rats

Figure 3. Effects of tomatidine on the viability of primary chondrocytes. (A) Chemical structure of tomatidine. (B, C) CCK-8 assay shows the viability of primary chondrocytes treated for 24 h with (B) 2.5, 5, 10 or 20 μM tomatidine alone or in combination with 10 ng/mlIL-1β (C). DMSO was used as control. As shown, treatments with 2.5, 5, or 10μM tomatidine or 10ng/mlIL-1β do not affect cell viability. Viability of primary chondrocytes is significantly affected by treatment with 20μM tomatidine in presence or absence of 10ng/mlIL-1β. The values are shown as means ± SD. *p < 0.05compared with the control group.