Research Paper Volume 12, Issue 14 pp 14037—14049

Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma

Figure 5. CNN3 silencing inhibits cell migration and invasion in vitro. (A, B) Cell migration and invasion were evaluated by a transwell assay (A) and the Transwell-Matrigel assay (B). Images on the left are representations, and results on the right are the statistical results of cell migration or invasion, where *p<0.05. (C) Wound healing assay to evaluate cell migration. Images on the left are representations, and results on the right are the statistical results of wound closure percentage. The wound area was analyzed using the Image Pro-Plus 6.0 software. The percentage of wound closure was calculated using the ratio of wound area at 0 h and 24 h. (D) Expression of tumor metastasis-related proteins MMP9, VEGF, vimentin, and E-cadherin was detected by western blotting. *p <0.05, when LV-shCNN3 vs LV-NC.