Research Paper Volume 12, Issue 12 pp 11500—11516

Long noncoding RNA LINC00963 induces NOP2 expression by sponging tumor suppressor miR-542-3p to promote metastasis in prostate cancer

Figure 4. Effects of tumor suppressor miR-542-3p on PCa cell metastasis in vitro and in vivo. (A) RT-qPCR analysis of miR-542-3p expression in the PCa cell lines DU 145 and PC-3 compared to that of the normal prostate cell line RWPE-1. (B) MiR-542-3p expression in OE-miR-542-3p Lentivirus- and empty lentivirus vector-transfected DU145 was tested by RT-qPCR. (C) Transwell invasion assays were performed to determine the invasion of OE-miR-542-3p Lentivirus– or empty lentivirus vector–transfected DU 145 cells. (D) A representative fluorescence image of miR-542-3p expression (green) and DAPI (blue) in OE-miR-542-3p-GFP- or empty lentivirus vector-infected prostate of PCa mice. Scale bars = 20 μm. (E) RT-qPCR analysis of miR-542-3p expression in OE-miR-542-3p Lentivirus– or empty lentivirus vector–transfected PCa tissues. (F) Survival time of PCa mice with OE-miR-542-3p Lentivirus– or empty lentivirus vector–transfected. (G) Experimental approach to knock down miR-542-3p in castrated TRAMP mice. (H) Incidence of TdLNs, liver, lung, and bone metastases following OE-miR-542-3p Lentivirus–transfection or pcDNA-miR-542-3p lentivirus–transfection of castrated TRAMP mice (n = 10, every group). (I) Experimental approach to knock down miR-542-3p in castrated ProbCre/Ptenfl/fl mice. (J) Incidence of TdLNs, liver, lung, and bone metastases following OE-miR-542-3p Lentivirus–transfection or pcDNA-miR-542-3p lentivirus–transfection in castrated ProbCre/Ptenfl/fl mice (n = 10, every group). Mean ± SEM, ****P < 0.001.