Figure 2. Ubiquitin-conjugating enzyme E2T (UBE2T) expression is higher in glioblastoma (GBM) and it promotes cell invasion and migration. (A) Immunohistochemical staining of UBE2T in human GBM tissues compared with the findings in normal brain tissues (×100). Scale bar, 100 μm. (B) Immunoblotting of UBE2T protein levels in normal human astrocytes (HEB and SVG p12) and GBM cell lines (U87, U118, LN229, U251 and U343). Expression levels were normalized to those of β-actin. (C) Immunoblotting of UBE2T protein levels in normal brain and GBM tissues. Expression levels were normalized to those of GAPDH. (D) Transfection efficacy of UBE2T short hairpin RNA (shRNA) in LN229 and U251 cells was analysed via immunoblotting. (E, F) The effects of UBE2T shRNA on the invasion and migration of LN229 (E) and U251 (F) cells. (Error bars indicate the SEM of three independent experiments. Two-tailed Student’s t-test. *, P < 0.05; **, P < 0.01; ***, P < 0.001).