Research Paper Volume 12, Issue 11 pp 10359—10369

Resveratrol promotes osteogenesis and alleviates osteoporosis by inhibiting p53

Figure 7. Resveratrol partially reverses p53-induced inhibition of osteogenic differentiation. (A) Effects of different concentrations of resveratrol on MDM2 expression were measured by qRT-PCR. (B) p53 expression in different groups was assessed by qRT-PCR. (C, D) Osteogenic genes expression in different groups was measured by qRT-PCR and western blot. (E) MDM2 expression in hMSCs after different treatments was assessed by qRT-PCR. (F) p53 expression in different groups was assessed by qRT-PCR. (GH) Alizarin red-mediated calcium staining in hMSCs 21 days after transfection with different constructs. Scale bar = 10mm. Data are means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.