Research Paper Volume 12, Issue 11 pp 10381—10397

High circ-SEC31A expression predicts unfavorable prognoses in non-small cell lung cancer by regulating the miR-520a-5p/GOT-2 axis

Figure 7. The relationships among miR-520a-5p, circ-SEC31A, and GOT2. (A) A schematic model showing the putative binding sites of 12 predicted miRNAs on circ-SEC31A. (B) Luciferase activity of circ-SEC31A in HEK293T cells transfected with miRNA mimics, which putatively bind to the circ-SEC31A sequence. Luciferase activity was normalized to Renilla luciferase activity. (C) The predicted binding sites of miR-520a-5p in circ-SEC31A. The mutated (Mut) version of circ-SEC31A is also shown. (D) Relative luciferase activity was determined 48 h after transfection with miR-520a-5p mimic/normal control (NC) or with the circ-SEC31A wild-type/Mut in HEK293T cells. Data are presented as mean ± SD; ***P<0.001. (E) A significant negative correlation between circ-SEC31A and miR-520a-5p was detected in NSCLCs tissues; n=20, P=0.0114. (F) RT-qPCR showing the relative expression of miR-520a-5p from 20 NSCLC tumor tissues and adjacent non-tumor tissues. Data are presented as mean ± SD; ***P<0.001. (G) The predicted binding sites of miR-520a-5p within the 3′-UTR of GOT2. The mutated version of the 3′-UTR of GOT2 is also shown. (H) Relative luciferase activity was determined 48 h after transfection with miR-520a-5p mimic/normal control or with the 3′-UTR-GOT2 wild-type/Mut in HEK293T cells. Data are presented as mean ± SD; ***P<0.001.