Research Paper Volume 12, Issue 11 pp 10415—10426

CHK2 is essential for spindle assembly and DNA repair during the first cleavage of mouse embryos

Figure 6. Inhibition of CHK2 induced autophagy during early embryonic development in mice. (A) Embryos at the 2-cell stage were immunolabeled with anti-LC3 antibody (green), and Hoechst 33342 was used to label DNA (blue). Bar = 30 μm. (B) The fluorescence intensity of LC3 in embryos in the 25 μM treatment group and control group. (C) Embryos at the 4-cell stage in the control and CHK2 inhibition groups were immunolabeled with anti-LC3 antibody (green), and Hoechst 33342 was used to label DNA (blue). Bar = 30 μm. (D) The fluorescence intensity of LC3 in embryos in the 25 μM treatment group and control group. (E) The protein expression of LC3-I/II in embryos at the 2-cell and 4-cell stages in the control and CHK2 inhibition groups. (F) Band intensity analysis of LC3-I/II in embryos in the control and CHK2 inhibition groups. (G) The expression of autophagy-related genes in the 25 μM and control groups. ***significant difference (p < 0.001). **significant difference (p < 0.01). *significant difference (p < 0.05).