Research Paper Volume 12, Issue 11 pp 10441—10456

LncRNA SNHG7 promotes cardiac remodeling by upregulating ROCK1 via sponging miR-34-5p

Figure 2. Silencing of SNHG7 inhibited cardiac remodeling after MI. (A) qRT-PCR analysis showing that AAV9-sh-SNHG7 reversed the up-regulation of SNHG7 in MI mice; GAPDH mRNA served as an internal control, and AAV9-sh-scramble served as an additional control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=6 mice per group. (B, C) 4 weeks after MI, echocardiographic findings showed that the silencing of SNHG7 improved ejection fraction (EF) and fraction shortening (FS). Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=12 mice per group. (D) mRNA expression of collagen 1α1 and collagen 3α1 were measured by qRT-PCR; GAPDH mRNA served as an internal control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=10 mice per group. (E) Western blotting analysis showing elevation of protein levels of collagen 1 and α-SMA in peri-infarcted tissue of left ventricle of mice after MI. Data was presented mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 mice per group. **P<0.05.