Research Paper Advance Articles

Human amnion-derived mesenchymal stem cells promote osteogenic differentiation of human bone marrow mesenchymal stem cells via H19/miR-675/APC axis

Figure 4. MiR-675 in HAMSCs is activated by lncRNA-H19 and promotes osteogenic differentiation of HBMSCs. (A) Transfection efficacy of miR-675 was detected by RT-PCR. (B) MiR-675 expression in HAMSCs during coculturing was measured by RT-PCR. (C) MiR-675 expression was measured by RT-PCR in NC, H19, shNC, and shH19 groups. (DF) Protein levels of ALP, RUNX2, OCN and OSX were assessed by western blot assay in NC, mimics, iNC and inhibitor groups. (G, H) Relative mRNA expressions of ALP, RUNX2, OCN, and OSX were measured by RT-PCR analysis in NC, mimics, iNC and inhibitor groups. (I) ALP staining and activity in NC, mimics, iNC and inhibitor groups. Scale bar, 100 μm. (J) Alizarin red staining and quantification in NC, mimics, iNC and inhibitor groups. Scale bar, 1 cm. Data are shown as mean ± SD. *P < 0.05 and **P < 0.01.