Research Paper Volume 12, Issue 11 pp 10544—10555

Long noncoding RNA LINC00460 conduces to tumor growth and metastasis of hepatocellular carcinoma through miR-342-3p-dependent AGR2 up-regulation

Figure 2. Knockdown of LINC00460 suppresses HCC cell proliferation, migration and invasion. Specific si-LINC00460 was transfected into HuH7 and SNU423 cells, respectively. si-NC was used as a blank. (A) Relative expression of LINC00460 in HuH7 and SNU423 cells was detected through qRT-PCR. (B, C) Bromodeoxyuridine assay was used to value cell proliferation in HuH7 and SNU423 cells, respectively. (D, E) Wound healing assay was conducted to value cell migration ability. Data statistics was also shown. (F, G) Transwell invasion assay showed the invasive cells in each group. Data statistics was also shown. (H, I) Western blot showed the expression of cell proliferation related proteins CyclinD1 and CDK4 and cell migration related proteins MMP-3 and MMP-9 in HuH7 cells. Data statistics was also shown. (J, K) Western blot showed the expression of CyclinD1, CDK4, MMP-3 and MMP-9 in SNU423 cells. Data statistics was also shown. (L) Flow cytometry was conducted to value cell apoptosis. (M) Cell apoptosis rate was calculated and presented. **P < 0.01, ***P < 0.001 vs the control group.