Research Paper Volume 12, Issue 11 pp 10544—10555

Long noncoding RNA LINC00460 conduces to tumor growth and metastasis of hepatocellular carcinoma through miR-342-3p-dependent AGR2 up-regulation

Figure 3. LINC00460 interacts with miR-342-3p in HCC cells. (A) Bioinformatics prediction showed the complementary sequence between LINC00460 and miR-342-3p. (B) Relative expression of miR-342-3p in HuH7 cells was detected through qRT-PCR. (C) Luciferase reporter assay was conducted to verify the targeting relationship between LINC00460 and miR-342-3p. (D) Relative expression of miR-342-3p in HuH7 cells was detected through qRT-PCR. (E, F) Western blot showed the expression of CyclinD1, CDK4, MMP-3 and MMP-9 in SNU423 cells. Data statistics was also shown. (G, H) Western blot showed the expression of Bax and Bcl-2 in SNU423 cells. Data statistics was also shown. *P < 0.05, **P < 0.01vs the control group. #P < 0.05, ##P < 0.01 vs the si- LINC00460 group.