Research Paper Volume 12, Issue 11 pp 10578—10593

CARM1 promotes non-small cell lung cancer progression through upregulating CCNE2 expression

Figure 1. CCNE2 promotes NSCLC cell proliferation in vitro. (A) The knockdown of CCNE2 by siRNAs in PC9 and HCC827 cells was verified by Western blot. GAPDH was used as loading control. (B) Cell proliferation abilities of CCNE2-depleted PC9 and HCC827 cells were assessed by CCK-8 assays. The data were presented as means ± SDs of three independent experiments; **P < 0.01. (C) Colony-formative abilities of CCNE2-depleted PC9 and HCC827 cells were determined by colony-formation assays. Right panel, the relative colony-formative abilities (% of NC) were quantified. The data were shown as means ± SDs of three independent experiments; **P < 0.01. (D) Overexpression of CCNE2 in PC9 and HCC827 cells was examined by Western blot. GAPDH was used as loading control. (E) Cell proliferation capacities of CCNE2-overexpressed PC9 and HCC827 cells were assessed by CCK-8 assays. The data were presented as means ± SDs of three independent experiments; **P < 0.01. (F) Colony-formative abilities of CCNE2-overexpressed PC9 and HCC827 cells were determined by colony-formation assays. Right panel, the relative colony-formative abilities (% of NC) were quantified. The data were shown as means ± SDs of three independent experiments; **P < 0.01.