Research Paper Volume 12, Issue 11 pp 10594—10613

Coagulation factor 2 thrombin receptor promotes malignancy in glioma under SOX2 regulation

Figure 2. Effects of F2R knocking down or overexpression on the glioma cell growth. (A, B) Western blotting and RT-qPCR analysis of the F2R expression in U87 and U251 cell lines stably silencing F2R. Sh-NC represents lentivirus-mediated control groups. Sh-F2R represents lentivirus-mediated F2R silencing groups. (C) The cell proliferation assays showed that knocking down F2R suppressed the growth rates of U87 and U251 cell lines. (D, E) Colony formation assay demonstrated that knocking down F2R suppressed the colony formation ability of U87 and U251 cell lines. (F, G) Western blotting and RT-qPCR analysis of the F2R expression in U87 and U251 cell lines transfected with F2R overexpression or control plasmid. (H) The cell proliferation assays showed that overexpression of F2R promoted the growth rates of U87 and U251 cell lines. (I, J) Colony formation assay demonstrated that overexpression of F2R promoted the colony formation ability of U87 and U251 cell lines. (K) Macroscopical view of the xenograft tumors. (L) Tumor volume growth curves. (M) Average xenograft tumor weights. All experiments were repeated at least three times. Data are presented as the mean ± SEM. *P<0.05 and **P<0.01.