Research Paper Volume 12, Issue 11 pp 10594—10613

Coagulation factor 2 thrombin receptor promotes malignancy in glioma under SOX2 regulation

Figure 4. F2R promotes glioma cell epithelial-mesenchymal transition (EMT) in vitro. (A) GSEA enrichment plots showed that enrichment of metastasis-related pathways was associated with up-regulation of F2R. (B) Western blot analysis revealed that knocking down F2R inhibited the epithelial-mesenchymal transition process. (C) Immunofluorescence staining of E-cadherin and Vimentin in U87 and U251 cells with F2R knockdown. (D) Representative images of metastatic lung in each group. (E) Representative HE images of metastatic lung in each group. Scale bar, 100 mm. (F) lung metastasis areas in each group. All experiments were repeated at least three times. Data are presented as the mean ± SEM. *P<0.05.