Research Paper Volume 12, Issue 11 pp 10594—10613

Coagulation factor 2 thrombin receptor promotes malignancy in glioma under SOX2 regulation

Figure 7. F2R promotes the malignant behavior of glioma via Wnt signal pathway. (A) GSEA enrichment plots demonstrated that enrichment of MYC targets and WNT signal pathways was associated with up-regulation of F2R. (B and C) Western blot analysis of F2R, c-MYC and β-catenin expression in F2R silenced or overexpressed U87 cells. (D and E) mRNA expression of the β-catenin signal pathway downstream genes (AXIN2, SOX9, CD44 and CCND2) in F2R knockdown or overexpressed U87 cells, which were determined by qPCR. (F and G) Western blot analysis of F2R, c-MYC and β-catenin expression in the U87 and U251 cell lines transfected with SOX2 or/and si-F2R. All of the experiments were performed at least three times. Data are means ± SEM. *P<0.05, **P<0.01.