Figure 2. The effect of UA on CCl4-induced liver fibrosis-related indicators is related to NOX4. (A) Dual immunofluorescence staining of liver sections from mice in the control, CCl4 and UA groups for nuclei (DAPI, blue), aHSCs (α-SMA, green), and apoptosis (TUNEL, red), and the merged images are shown. (B) Hepatic mRNA levels of collagen I, MMP-1, α-SMA, and TIMP-1 were measured by qRT-PCR. (C) Collagen I, MMP-1, α-SMA, and TIMP-1 protein expression was detected by a western blot. Data represent the mean ± SD of each group. *P < 0.05 and ***P < 0.001.