Research Paper Volume 12, Issue 11 pp 10614—10632

Ursolic acid reverses liver fibrosis by inhibiting interactive NOX4/ROS and RhoA/ROCK1 signalling pathways

Figure 8. Effect of UA on intestinal microbiota dysbiosis in mice with CCl4-induced liver fibrosis. (A) Ileal mRNA levels of the TJ proteins ZO-1 and occludin were measured by qRT-PCR. (B) The alpha diversity of each group was assessed by determining the Shannon index and the Chao1 index. (C) PCoA to determine the weighted UniFrac distance of the intestinal microbiota. (D) Composition of the intestinal microbiota of each group at the phylum level. (E) Composition of the intestinal microbiota of each group at the genus level. (F) Linear discriminant analysis effect size (LEfSe) prediction was used to identify the bacteria in each group with the most differential abundance. (G) Linear discriminant analysis (LDA) scores showed significant differences in the bacteria in each group. Only the bacteria whose abundance met an LDA threshold value of >2 are shown. Data represent the mean ± SD of each group. *P < 0.05 and ***P < 0.001.