Research Paper Volume 12, Issue 12 pp 11530—11549

Hsa_circ_0085576 promotes clear cell renal cell carcinoma tumorigenesis and metastasis through the miR-498/YAP1 axis

Figure 4. Hsa_circ_0085576 promotes cell growth and metastasis of ccRCC in vivo. (AF) A, Tumor volumes of A498/LV-sh-hsa_circ_0085576 were measured every week for 4 weeks. B, Images of subcutaneous xenograft tumors of A498/LV-sh- hsa_circ_0085576 cells. C, the final tumor weight of A498/LV-sh-hsa_circ_0085576 cells was shown. D, Tumor volumes of 786O/pLVX-hsa_circ_0085576 cells were measured every week for 4 weeks. E, Images of subcutaneous xenograft tumors of 786O/pLVX-hsa_circ_0085576 cells. F, the final tumor weight of 786O/pLVX-circ0085567 cells was shown. (G, H) the expression of hsa_circ_0085576 was detected by RT-qPCR analysis in tumors with A498/LV-sh-hsa_circ_0085576 or 786O/pLVX-hsa_circ_0085576. (I, J) Stably transfected A498 cells with LV-sh-hsa_circ_0085576 or 786O cells with pLVX-hsa_circ_0085576 were injected into the vein of BALB/c nude mice for 4 weeks. Representative images of lungs (metastatic nodules were indicated by arrows) and H&E staining of lung metastatic lesions was shown. The number of metastatic nodules and metastasis areas in the lungs of BALB/c nude mice is quantified for each group (n=6). * P<0.05 vs. LV-sh-Ctrl; ** P<0.05 vs. pLVX-Ctrl.