Research Paper Volume 12, Issue 12 pp 11530—11549

Hsa_circ_0085576 promotes clear cell renal cell carcinoma tumorigenesis and metastasis through the miR-498/YAP1 axis

Figure 8. The oncogenic hsa_circ_0085576/miR-498/YAP1 axis in ccRCC cells. (A) Western blot analysis of YAP1 expression levels in A498 cells and 786O cells transfected with LV-shCtrl, LV-sh-hsa_circ_0085576 miR-498 inhibitors or YAP1 plasmid. (B, C) CCK-8 assay was used to detect the cell proliferation of A498 cells and 786O cells with indicated treatment. (D, E) Transwell assay showed migration and invasion of A498 and 786O cells with indicated treatment. (F) flow cytometry was used to determine the apoptotic rates of A498 cells and 786O cells with indicated treatment. * P<0.05 vs. LV-shCtrl; ** P<0.05 vs. LV-sh-hsa_circ_0085576.