Research Paper Volume 12, Issue 11 pp 10931—10950

Hypoxia-preconditioned olfactory mucosa mesenchymal stem cells abolish cerebral ischemia/reperfusion-induced pyroptosis and apoptotic death of microglial cells by activating HIF-1α

Figure 3. Hypoxia-preconditioned OM-MSCs prevented cerebral OGD/R-induced apoptosis in BV2 microglial cells. (AC) Protein and mRNA expression of HIF-1α in OM-MSCs as determined by western-blot and qPCR, respectively. (DE) The apoptosis rate of BV2 microglial cells as evaluated by flow cytometry with Annexin V/PI staining. (F, G) ROS generation in BV2 microglial cells as measured by flow cytometry. (H, I) The expression of caspase3 in BV2 microglial cells as determined by Western blotting analysis. (J) The viability of BV2 microglial cells as determined by MTT assay. All data are presented as the mean value ±SD. *p<0.05; **p<0.01, ***p<0.001, compared with normoxia group.