Figure 3. Hypoxia-preconditioned OM-MSCs prevented cerebral OGD/R-induced apoptosis in BV2 microglial cells. (A–C) Protein and mRNA expression of HIF-1α in OM-MSCs as determined by western-blot and qPCR, respectively. (D–E) The apoptosis rate of BV2 microglial cells as evaluated by flow cytometry with Annexin V/PI staining. (F, G) ROS generation in BV2 microglial cells as measured by flow cytometry. (H, I) The expression of caspase3 in BV2 microglial cells as determined by Western blotting analysis. (J) The viability of BV2 microglial cells as determined by MTT assay. All data are presented as the mean value ±SD. *p<0.05; **p<0.01, ***p<0.001, compared with normoxia group.