Research Paper Volume 12, Issue 11 pp 10931—10950

Hypoxia-preconditioned olfactory mucosa mesenchymal stem cells abolish cerebral ischemia/reperfusion-induced pyroptosis and apoptotic death of microglial cells by activating HIF-1α

Figure 5. Knockdown of HIF-1α in OM-MSCs aggravated cerebral OGD/R-induced apoptosis in BV2 microglial cells under hypoxic conditions. (AC) Successful knockdown of HIF-1α in OM-MSCs was verified by western-blot and qPCR. (DF) The expression of HIF-1α in OM-MSCs was evaluated by western-blot and qPCR. (G, H) The apoptosis rate of BV2 microglial cells was evaluated by flow cytometry with Annexin V/PI staining. (I, J) Production of ROS in BV2 microglial cells was evaluated by flow cytometry. (K, L) The expression of caspase3 in BV2 microglial cells was quantified by Western blotting analysis. (M) The viability of BV2 microglial cells was assessed by MTT assay. All data are presented as the mean value ±SD. *p<0.05; **p<0.01, ***p<0.001, compared with vector group.