Figure 1. Mlxipl was upregulated in ipsilateral SDH of SNI-induced mechanical allodynia rats. (A and B) The Von Frey test were carried out before and after surgery (Sham or SNI). Mechanical allodynia was induced by SNI surgery in the ipsilateral paw. N = 5. ***P < 0.001 vs. SNI BL Ipsi; $$$P < 0.001, $$P < 0.01 vs. SNI Contra; ###P < 0.001 vs. Sham Ipsi. QPCR (C) and western blot (D) western blot (E) were performed before and after surgery. Mlxipl in the ipsilateral SDH was upregulated after SNI surgery. Quantification of the western blot (F). N = 5. ***P < 0.001, **P < 0.01 vs. SNI BL Ipsi; $$$P < 0.001, $$P < 0.01 vs. SNI BL Contra. (G) and immunofluorescence (I) were performed at day 7 after Sham or SNI surgery. Mlxipl was significantly upregulated in the ipsilateral SDH. Quantification of the western blot (H) and immunofluorescence (J). N = 5. $$$P < 0.001, $$P < 0.01 vs. SNI Contra; ###P < 0.001, ##P < 0.01 vs. Sham Ipsi. (K) Double immunofluorescence staining showed that Mlxipl was co-localized with Iba1 (white arrows) and NeuN (yellow arrows) but not GFAP. BL, baseline (before surgery); ipsi, ipsilateral; Contra, contralateral; SNI, spare nerve injury; SDH, spinal dorsal horn.