Figure 3. Overexpression of Mlxipl in the SDH inhibited mechanical allodynia and neuroinflammation. (A–E) Mlxipl in the ipsilateral SDH was overexpressed by intraspinal microinjection of OeMLXIPL. SNI surgery was performed on day 28 after intraspinal microinjection. QPCR (A), western blot (B) and immunofluorescence (D) were performed at day 7 after SNI surgery. Quantification of western blot (C) and immunofluorescence (E). N = 5. ***P < 0.001 vs. SNI Ipsi; ###P < 0.001 vs. SNI vehicle Ipsi; $$$P < 0.001 vs. SNI shNC Ipsi. (F and G) Overexpression of Mlxipl inhibited mechanical allodynia in the ipsilateral paw. The Von Frey test was performed before and after SNI surgery with or without pre-microinjection. N = 5. **P < 0.01, *P < 0.05 vs. SNI Ipsi 0D; ##P < 0.01, #P < 0.05 vs. SNI vehicle Ipsi 0D; $$P < 0.01, $P < 0.05 vs. SNI OeNC Ipsi 0D. (H–J) Overexpression of Mlxipl inhibited neuroinflammation in the ipsilateral SDH. Proinflammatory cytokines were detected using qPCR at day 7 after SNI surgery. N = 5. ***P < 0.001 vs. SNI Ipsi; ###P < 0.001 vs. SNI vehicle Ipsi; $$$P < 0.001 vs. SNI shNC Ipsi. BFMI, before microinjection; BL, baseline (before surgery); ipsi, ipsilateral; Contra, contralateral; SNI, spare nerve injury; SDH, spinal dorsal horn.