Research Paper Volume 12, Issue 11 pp 11025—11041

LncRNA ADAMTS9-AS2 inhibits gastric cancer (GC) development and sensitizes chemoresistant GC cells to cisplatin by regulating miR-223-3p/NLRP3 axis

Figure 4. The expression patterns of LncRNA ADAMTS9-AS2 and miR-223-3p were changed by long-term cisplatin stimulation in GC cells. Real-Time qPCR was used to examine the expression levels of (A) LncRNA ADAMTS9-AS2 and (B) miR-223-3p in CS-GC and CR-GC cells. (C) The schematic diagram for the production of ACR-GC cells involved in this study. (D) Trypan blue staining assay was performed to evaluate cell viability in CS-GC cells and ACR-GC cells. Real-Time qPCR was conducted to examine the expression levels of (E) LncRNA ADAMTS9-AS2 and (F) miR-223-3p in GC cells treated with continuous low-dose cisplatin stimulation. Each experiment repeated at least 3 times. “NS” represented “no statistical significance”, *P < 0.05, **P < 0.01.