Research Paper Volume 12, Issue 12 pp 11579—11602

Long-term PM2.5 exposure increases the risk of non-small cell lung cancer (NSCLC) progression by enhancing interleukin-17a (IL-17a)-regulated proliferation and metastasis

Figure 7. IL-17a treatment promotes the proliferation and EMT in NSCLC cells. (AG) A549 and H1350 cells were treated with or without IL-17a (100 ng/ml) for 24 h, and then were collected for the following studies. (A) CCK-8 analysis was used to determine the cell proliferative activity. (B) Colony formation and EdU assays were used to determine the cell proliferative condition. Scale bar, 100 μm. (C) Quantification of the number of cells by colony formation and EdU analysis. (D) Transwell analysis was used to calculate the number of cells in migration and invasion. Scale bar, 100 μm. (E) Quantification of the counts of the migrated and invaded cells. (F) RT-qPCR analysis was used to measure EMT-associated genes in A549 and H1350 cells. (G) IF staining of N-cadherin in A549 and H1350 cells. Scale bar, 50 μm. All data are expressed as mean ± SEM. *p<0.05 and **p<0.01 compared to the Ctrl group.