Research Paper Volume 12, Issue 12 pp 11623—11635

Figure 2. Downregulation of hsa_circ_0101145 suppressed HCC cell proliferation in vivo and in vitro. (A) RT-qPCR detection of hsa_circ_0101145 expression in HepG2 and Huh-7 cells following transfection with siRNA targeting hsa_circ_0101145 (si-circ0101145) or the negative control (NC). Data are presented as the mean ± SD. ^***P < 0.001 vs. NC. (B) Flow cytometry showing the percentages of cells in G1, S, or G2 phase in HepG2 and Huh-7 cells. (C and D) CCK-8 assay showing the proliferation of HepG2 (C) and Huh-7 (D) cells. Data are presented as the mean ± SD. ^**P < 0.01, ^***P < 0.001 vs. NC. (E and F) Colony formation assay showing proliferation of HepG2 and Huh-7 cells. Data are presented as mean ± SD. ^***P < 0.001 vs. NC. (G–J) Western blot analysis of the expression of E-cadherin (epithelial marker), and N-cadherin and vimentin (mesenchymal markers). Data are presented as the mean ± SD. ^***P < 0.001 vs. NC.