Research Paper Volume 12, Issue 12 pp 11623—11635

Silencing of hsa_circ_0101145 reverses the epithelial-mesenchymal transition in hepatocellular carcinoma via regulation of the miR-548c-3p/LAMC2 axis

Figure 6. Downregulation of miR-548c-3p or overexpression of LAMC2 restored the proliferation and migration abilities of both HepG2 and Huh-7 cells following silencing of hsa_circ_0101145. (AF) RT-qPCR analysis shows the expression of hsa_circ_0101145 (A and B), miR-548c-3p (C and D), and LAMC2 (E and F) in HepG2 and Huh-7 cells following transfection or treatment with NC, si-circ0101145, miR-548c-3p inhibitor, LAMC2 overexpression vector (LAMC2) single or combined. Data are presented as the mean ± SD. ***P < 0.001 vs. NC. ###P < 0.001 vs. si-circ0101145. (GI) Cell proliferation was analyzed by colony formation. Data are presented as the mean ± SD. ***P < 0.001 vs. NC. ###P < 0.001 vs. si-circ0101145. (JL) Cell migration was assessed in HepG2 and Huh-7 cells using Transwell assays. Data are presented as the mean ± SD. ***P < 0.001 vs. NC. ###P < 0.001 vs. si-circ0101145. (M and N) Western blot analysis of the expression of E-cadherin, N-cadherin, and vimentin.