Research Paper Volume 12, Issue 12 pp 11623—11635

Silencing of hsa_circ_0101145 reverses the epithelial-mesenchymal transition in hepatocellular carcinoma via regulation of the miR-548c-3p/LAMC2 axis

Figure 7. LAMC2 overexpression reversed miR-548c-3p-induced cell migration and growth inhibition in vitro. HepG2 and Huh-7 cells were transfected with miR-548c-3p mimics with or without the LAMC2 overexpression vector. (A and B) RT-qPCR assay showing the expression of miR-548c-3p (A and B) and LAMC2 (C and D) in HepG2 and Huh-7 cells. Data are mean ± SD. ***P < 0.001 versus NC. ###P < 0.001 versus mimic. (EG) Colony formation assay showing the proliferation of HepG2 and Huh-7 cells. Data are mean ± SD. ***P < 0.001. ###P < 0.001 versus mimic. (HJ) Cell migration were assessed in HepG2 and Huh-7 cells by Transwell assays. Data are mean ± SD. ***P < 0.001 versus NC. ###P < 0.001 versus mimic. (K and L) Western blot analysis of the expression of E-cadherin, N-cadherin, and vimentin. Data are mean ± SD. ***P < 0.001 vs. NC. ###P < 0.001 vs. the mimic.