Research Paper Volume 12, Issue 11 pp 11100—11115

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L

Figure 2. ANXA7 overexpression promotes the proliferation of U266 and RPMI8226 cells. (A) The expression of ANXA7 in U266 cells after transfection of OE-ANXA7 was detected by Western blot and RT-qPCR analysis. *P<0.05 and ***P<0.001 vs. Control group. #P<0.05 and ###P<0.001 vs. OE-NC group. (B) The expression of ANXA7 in RPMI8226 cells after transfection of OE-ANXA7 was detected by Western blot and RT-qPCR analysis. *P<0.05 and ***P<0.001 vs. Control group. #P<0.05 and ###P<0.001 vs. OE-NC group. (C) The proliferation of U266 and RPMI8226 cells after transfection of OE-ANXA7 was determined by CCK-8 assay. **P<0.01 vs.U266 group. #P<0.05 and ##P<0.01 vs. U266+OE-NC group. ΔΔΔP<0.001 vs. RPMI8226 group. $$$P<0.001 vs. RPMI8226-OE-NC group. (D) The proliferation of U266 and RPMI8226 cells after transfection of OE-ANXA7 was also showed by colony formation assay. (E) The protein expression of PCNA and KI67 in U266 and RPMI8226 cells after transfection of OE-ANXA7 was detected by Western blot analysis. ***P<0.001 vs.U266 group. ###P<0.001 vs. U266-OE-NC group. ΔΔΔP<0.001 vs. RPMI8226 group. $$$P<0.001 vs. RPMI8226-OE-NC group.