Research Paper Volume 12, Issue 11 pp 11100—11115

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L

Figure 4. ANXA7 interference inhibits the proliferation of U266 and RPMI8226 cells. (A) The expression of ANXA7 in U266 cells after transfection of shRNA-ANXA7 was detected by Western blot and RT-qPCR analysis. **P<0.01 and ***P<0.001 vs. Control group. ##P<0.01 and ###P<0.001 vs. shRNA-NC group. ΔΔΔP<0.001 vs. shRNA-ANXA7-1 group. (B) The expression of ANXA7 in RPMI8226 cells after transfection of shRNA- ANXA7 was detected by Western blot and RT-qPCR analysis. ***P<0.001 vs. Control group. ###P<0.001 vs. shRNA-NC group. ΔP<0.05 vs. shRNA-ANXA7-1 group. (C) The proliferation of U266 and RPMI8226 cells after transfection of shRNA- ANXA7 was determined by CCK-8 assay. *P<0.05 vs.U266 group. #P<0.05 vs. U266-shRNA-NC group. ΔΔP<0.01 vs. RPMI8226 group. $P<0.05 vs. RPMI8226-shRNA-NC group. (D) The proliferation of U266 and RPMI8226 cells after transfection of shRNA- ANXA7 was also showed by colony formation assay. (E) The protein expression of PCNA and KI67 in U266 and RPMI8226 cells after transfection of shRNA-ANXA7 was detected by Western blot analysis. ***P<0.001 vs.U266 group. ###P<0.001 vs. U266-shRNA-NC group. ΔΔΔP<0.001 vs. RPMI8226 group. $$$P<0.001 vs. RPMI8226-shRNA-NC group.