Research Paper Volume 12, Issue 11 pp 11100—11115

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L

Figure 5. ANXA7 interference leads to G2/M arrest of cell cycle. (A) The images of flow cytometry for U266 and RPMI8226 cells after transfection. (B) The cell cycle distribution of U266 and RPMI8226 cells after transfection of shRNA-ANXA7 was analyzed by flow cytometry analysis. **P<0.01 vs.U266 group. ##P<0.01 vs. U266-shRNA-NC group. ΔΔΔP<0.001 vs. RPMI8226 group. $P<0.05 and $$$P<0.001 vs. RPMI8226-shRNA-NC group. (C) The protein expression of CDK1 and cyclinB1 in U266 and RPMI8226 cells after transfection of shRNA-ANXA7 was detected by Western blot analysis. *P<0.05 vs.U266 group. #P<0.05 vs. U266-shRNA-NC group. ΔΔΔP<0.001 vs. RPMI8226 group. $$$P<0.001 vs. RPMI8226-shRNA-NC group.