Research Paper Volume 12, Issue 11 pp 11100—11115

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L

Figure 9. CDC5L interference inhibits the proliferation promotion effect of ANXA7. (A) The expression of CDC5L in U266 cells after transfection of shRNA-CDC5L was detected by Western blot and RT-qPCR analysis. *P<0.05 and ***P<0.001 vs. Control group. #P<0.05 and ###P<0.001 vs. shRNA-NC group. ΔP<0.05 and ΔΔP<0.01 vs. shRNA-CDC5L-1 group. (B) The expression of CDC5L in RPMI8226 cells after transfection of shRNA-CDC5L was detected by Western blot and RT-qPCR analysis. **P<0.01 and ***P<0.001 vs. Control group. #P<0.05, ##P<0.01 and ###P<0.001 vs. shRNA-NC group. ΔP<0.05 vs. shRNA-CDC5L-1 group. (C) The proliferation of U266 and RPMI8226 cells after transfection of shRNA-CDC5L was determined by CCK-8 assay. **P<0.01 vs.U266 group. #P<0.05 vs. U266-OE-ANXA7 group. ΔΔΔP<0.001 vs. RPMI8226 group. $$P<0.01 and $$$P<0.001 vs. RPMI8226-OE-ANXA7 group. (D) The proliferation of U266 and RPMI8226 cells after transfection of shRNA-CDC5L was also showed by colony formation assay. (E) The protein expression of PCNA and KI67 in U266 and RPMI8226 cells after transfection of shRNA-CDC5L was detected by Western blot analysis. **P<0.01 and ***P<0.001 vs.U266 group. #P<0.05 and ###P<0.05 vs. U266-OE-ANXA7 group. ΔΔP<0.01 and ΔΔΔP<0.001 vs. RPMI8226 group. $$$P<0.001 vs. RPMI8226-OE-ANXA7 group.