Research Paper Volume 12, Issue 11 pp 11116—11138

Deoxyelephantopin induces apoptosis via oxidative stress and enhances gemcitabine sensitivity in vitro and in vivo through targeting the NF-κB signaling pathway in pancreatic cancer

Figure 7. DET amplified the effect of GEM to inhibit the growth of pancreatic cancer in vivo. (A) The specific grouping strategies and treatment patterns of subcutaneous tumor model. (B) Xenograft tumors were established by subcutaneous injection of BxPC-3 cells (n = 4). (C, D) Curves of subcutaneous tumor volume in nude mice. **P < 0.01 versus CTL. ##P < 0.01 versus GEM monotherapy group. CTL, control. (E) The final quantitative statistics of tumor weight. **P < 0.01 versus CTL. ##P < 0.01 versus GEM monotherapy group. CTL, control. (F) Ki-67, PCNA and E-cadherin staining and quantitative statistics of the xenograft tumors were shown. *P < 0.05, **P < 0.01 versus CTL. #P < 0.05 versus GEM monotherapy group. CTL, control. (G) EMT-related protein levels in tumor tissues were detected and quantified by western blotting. Quantitative statistics of western blotting analysis for Snail levels (H), E-cadherin levels (I) and N-cadherin levels (J). *P < 0.05, **P < 0.01 versus CTL. #P < 0.05, ## P < 0.01 versus GEM monotherapy group. CTL, control. Magnification, × 200 (F). Scale bar, 100 μm (F).