Research Paper Volume 12, Issue 12 pp 11685—11697

Long noncoding RNA KCNQ1OT1 promotes colorectal carcinogenesis by enhancing aerobic glycolysis via hexokinase-2

Figure 2. KCNQ1OT1 silencing inhibits proliferation of colorectal cancer cells. (A) QRT-PCR analysis shows KCNQ1OT1 levels in SW48, LoVo, HCT116, SW620, HT-29 and RKO colorectal cancer cell lines. (B) QRT-PCR analysis shows KCNQ1OT1 levels in sh-NC- and sh-KCNQ1OT1-transfected HCT 116 and SW48 CRC cell lines. (C, D) CCK8 assay results show proliferation status of sh-NC- and sh-KCNQ1OT1-transfected HCT 116 and SW48 CRC cell lines. (E) Representative images show colony formation assay results in sh-NC- and sh-KCNQ1OT1-transfected HCT 116 and SW48 CRC cell lines. (F) Histogram plot shows total number of colonies in sh-NC- and sh- KCNQ1OT1-transfected HCT 116 and SW48 CRC cell lines. (G, H) Cell cycle analysis results of Sh-NC- and Sh- KCNQ1OT1-transfected HCT 116 and SW48 CRC cell lines is shown through the flow cytometry analysis. Note: ** denotes p < 0.01 and *** denotes p < 0.001.