Research Paper Volume 12, Issue 12 pp 11685—11697

Long noncoding RNA KCNQ1OT1 promotes colorectal carcinogenesis by enhancing aerobic glycolysis via hexokinase-2

Figure 3. KCNQ1OT1 silencing inhibits aerobic glycolysis in colorectal cancer cells. (A, B) ECAR assay results show extracellular acidification rate of sh-NC- and sh-KCNQ1OT1-transfected HCT116 and SW48 CRC cell lines. Each data point represents means ± SD. (C, D) Lactate assay results show the levels of lactate in the media of sh-NC- and sh-KCNQ1OT1-transfected HCT116 and SW48 CRC cell lines. (E, F) Glucose assay results show the glucose levels in the media of sh-NC- and sh-KCNQ1OT1-transfected HCT116 and SW48 CRC cell lines. (G, H) Metabolic labeling assay results show the ratio of 13C-glucose vs. unlabeled glucose in sh-NC- and sh-KCNQ1OT1-transfected HCT116 and SW48 CRC cell lines. (I) CCK-8 assay results show the proliferation status of 2-deoxyglucose-treated control and KCNQ1OT1-overexpressing LoVo cells. Note: *p < 0.05, **p < 0.01, ***p < 0.001.