Research Paper Volume 12, Issue 16 pp 16021—16034

Long non-coding RNA DLX6-AS1 facilitates bladder cancer progression through modulating miR-195-5p/VEGFA signaling pathway

Figure 4. DLX6-AS1 targets miR-195-5p. (A) The expression of DLX6-AS1 in BC cells was determined by FISH assay. (B) The specific location of DLX6-AS1 was identified in BC cells through performing subcellular fractionation assay. (C) The binding sites of DLX6-AS1 and miR-195-5p was predicted. (D) The binding of miR-195-5p and DLX6-AS1 was verified by dual-luciferase reporter assay. (E) The binding of miR-195-5p and DLX6-AS1 was validated by RIP assay. (F) The binding of miR-195-5p and DLX6-AS1 was validated by RNA pull down assay. (G) The expression of miR-195-5p in BC cells with DLX6-AS1 overexpression or knockdown was determined by qRT-PCR. (H) The expression of miR-195-5p in BC tissues and the relationship between DLX6-AS1 and miR-195-5p expression were determined. (I) The miR-195-5p expressions in BC cell lines (T24, RT4, 5637, J82, and SW780) and SV-HUC-1 cells by qRT-PCR. Data were expressed as the mean ± SD, n = 3. ***P < 0.001.