Research Paper Volume 12, Issue 12 pp 12160—12174

ROCK1 knockdown inhibits non-small-cell lung cancer progression by activating the LATS2-JNK signaling pathway

Figure 1. ROCK1 regulates A549 cell viability. (A) MTT assay for A549 cells. A549 cells were transfected with siRNA against ROCK1 (si/ROCK1) or control siRNA (si/Ctrl). (B) An LDH release assay was used to measure LDH levels in the medium of A549 cells transfected with siRNA against ROCK1 (si/ROCK1) or control siRNA (si/Ctrl). (C) ELISA was used to analyze Caspase-3 activity in response to si/ROCK1 or si/Ctrl transfection. (D) A qPCR assay was used to measure Caspase-3 transcription. (E, F) TUNEL staining was used to measure numbers of apoptotic cells in response to si/ROCK1 or si/Ctrl transfection. *p<0.05.