Research Paper Volume 12, Issue 20 pp 20069—20084

LncRNA FAM181A-AS1 promotes gliomagenesis by sponging miR-129-5p and upregulating ZRANB2

Figure 5. LncRNA FAM181A-AS1 sponges miR-129-5p in glioma cells. (A) The diagrammatic representation shows miR-129-5p-binding sites in the wild-type and mutant FAM181A-AS1sequences. (B) Dual luciferase reporter assay results show relative firefly luciferase activity in U87 and U251 cell lines transfected with miR-129-5p mimic and vectors containing FAM181A-AS1 with wild-type (WT) or mutant (MUT) miR-129-5p-binding sites. (C) QRT-PCR analysis shows miR-129-5p levels in control and miR-129-5p mimic-transfected U87 and U251 cell lines. (D) QRT-PCR analysis shows miR-129-5p levels in control and FAM181A-AS1 overexpressing U87 and U251 cell lines. (E) Ago2 RIP assay results show enrichment of FAM181A-AS1 and miR-129-5p using qRT-PCR in Ago2 protein pulldown samples from U87 and U251 cell lines using anti-Ago2 antibody. (F) QRT-PCR assay shows the levels of miR-129-5p in the RNA-RNA complexes pulled down using the FAM181A-AS1 biotinylated probe in comparison with the negative control. Note: **P<0.01, ***P<0.001; data are represented as means ± SD.