Research Paper Volume 12, Issue 20 pp 20069—20084

LncRNA FAM181A-AS1 promotes gliomagenesis by sponging miR-129-5p and upregulating ZRANB2

Figure 8. ZRANB2 overexpression promotes proliferation of FAM181A-AS1 downregulating glioma cells. (A) Transfection efficiency of ZRANB2-expression plasmid in LN229 and A172 cell lines is examined through qRT-PCR. (B, C) CCK-8 and EdU analyses of the cell viability after transfection with FAM181A-AS1 siRNA and (or) ZRANB2-expression plasmid in LN229 and A172 cell lines. (D) Colony formation analysis of the cell colony abilities after transfection with FAM181A-AS1 siRNA plasmid and (or) ZRANB2-expression plasmid in LN229 and A172 cell lines. **P<0.01, ***P<0.001, ns: no statistical significance, data represent the mean ± SD.