Figure 2. Effect of AKR1B10 on CRC cell proliferation and migration ability. (A) Comparison of AKR1B10 mRNA expression in CRC and normal tissues across 7 Oncomine datasets. (B–C) AKR1B10 mRNA levels in (B) 27 paired CRC and normal tissues and (C) 5 CRC cell lines. (D–E) Immunoblots showing AKR1B10 protein levels in (D) wild type and (E) AKR1B10-KD and AKR1B10-OE CRC cell lines. (F–H) Proliferation rates (F), colony forming capacity (G) and migration rates (H) of AKR1B10-KD and AKR1B10-OE CRC cells. CRC, colorectal cancer. CTL, control; NC, negative control; KD, AKR1B10-shRNA; VEC, vector; OE, AKR1B10 overexpression plasmid. Data are presented as mean ± SD (n=3). *P < 0.05, **P < 0.01, ***P < 0.001.