Figure 2. GLSO has an obvious impact on the collagen deposition on day 5. (A) Masson’s Trichrome was used to stain collagen deposition. Typical images of the stained tissue sections from the model group and GLSO group were from different healing time points under the microscope 40X (Scale bar = 1 mm). (B) Quantitation results of Masson’s Trichrome staining. (C, D) Immunohistochemical analysis of collagen I (C) and collagen III (D) at the murine wound edge from the model group and GLSO group under the microscope 100X (scale bar = 400 μm). (E, F) Quantitation results of collagen I (E) and collagen III (F) staining in comparison between the model group and GLSO group (n=3 each group).