Research Paper Volume 12, Issue 14 pp 14141—14156

Long noncoding RNA AC092171.4 promotes hepatocellular carcinoma progression by sponging microRNA-1271 and upregulating GRB2

Figure 5. AC092171.4 regulates GRB2 protein expression by competitively binding miR-1271. (A) QRT-PCR analysis shows miR-1271 levels in sh-NC and sh-AC092171.4-transfected HCC cells. (B) QRT-PCR analysis shows miR-1271 levels in control and AC092171.4 overexpressing HCC cells. (C) Dual luciferase reporter assay results show relative firefly luciferase activity in HCC cells transfected with wild-type or mutant AC092171.4 and miR-1271 mimics. (D) Pearson’s correlation analysis shows the association between AC092171.4 and miR-1271 levels in 45 HCC tissue samples from 70 pair HCC specimens. (E) Dual luciferase reporter assay results show relative firefly luciferase activity in HCC cells transfected with WT or mutant 3’UTR of GRB2 and miR-1271 mimics. (F) Western blot results show GRB2 protein levels in HCC cells transfected with miR-1271 mimics or miR-1271 inhibitors. (G) Pearson’s correlation analysis shows the relationship between AC092171.4 and GRB2 mRNA levels in HCC tissues from the TCGA datasets in GEPIA website. (H) Pearson’s correlation analysis shows the relationship between AC092171.4 and GRB2 mRNA levels in HCC tissues from the TCGA datasets. (I) Western blot analysis shows GRB2 protein expression in HCC cells transfected with AC092171.4-shRNA plus miR-1271 inhibitor or AC092171.4-shRNA alone. * denotes p<0.05.