Research Paper Volume 12, Issue 14 pp 14141—14156

Long noncoding RNA AC092171.4 promotes hepatocellular carcinoma progression by sponging microRNA-1271 and upregulating GRB2

Figure 6. AC092171.4 promotes proliferation, migration, and invasiveness of HCC cells by suppressing miR-1271-dependent downregulation of GRB2 protein translation. (A) CCK-8 assay results show proliferation of Huh-7 and LM3 cells transfected with sh-NC, sh-AC092171.4, sh-NC plus miR-1271 inhibitor, or sh-AC092171.4 plus miR-1271 inhibitor. (B) Colony formation assays show the numbers of colonies formed by Huh-7 cells transfected with sh-NC, sh-AC092171.4, sh-NC plus miR-1271 inhibitor, or sh-AC092171.4 plus miR-1271 inhibitor. (C) Transwell assay results show the total numbers of migratory and invasive Huh-7 cells transfected with sh-NC, sh-AC092171.4, sh-NC plus miR-1271 inhibitor, or sh-AC092171.4 plus miR-1271 inhibitor. (D) Colony formation assays show the numbers of colonies formed by LM3 cells transfected with sh-NC, sh-AC092171.4, sh-NC plus miR-1271 inhibitor, or sh-AC092171.4 plus miR-1271 inhibitor. (E) Transwell assay results show the total numbers of migratory and invasive LM3 cells transfected with sh-NC, sh-AC092171.4, sh-NC plus miR-1271 inhibitor, or sh-AC092171.4 plus miR-1271 inhibitor. (F) Western blot analysis shows relative levels of E-cadherin, N-cadherin and vimentin levels in HCC cells transfected with shRNA-AC092171.4 alone or shRNA-AC092171.4 plus miR-1271 inhibitor. (G) CCK-8 assay results show the proliferation status in AC092171.4-silenced and sh-AC092171.4-silenced plus GRB2 overexpressing Huh7and LM3 cells. (H, I) Transwell assay results show the migration status of AC092171.4-silenced and sh-AC092171.4-silenced plus GRB2 overexpressing Huh7and LM3 cells. * denotes p<0.05.