Research Paper Volume 12, Issue 12 pp 12305—12323

Figure 2. BMP3 expression was significantly downregulated in AIA. (A) Representative H&E staining of normal and AIA rat synovial tissues (original magnification, ×20). (B) BMP3 expression in normal and AIA rat synovial tissues was analyzed using IHC staining (original magnification, ×20). (C) BMP3 expression in normal and AIA rat synovial tissues was analyzed using immunofluorescence staining (original magnification, ×20). (D) BMP3 expression in FLS from AIA rats treated with TNF-α was analyzed using immunofluorescence staining (original magnification, ×10). (E) BMP3 protein levels in normal and AIA synovial tissues were analyzed using western blot. (F) BMP3 protein levels in AIA FLS treated with various inflammation factors were analyzed using western blot. All values are expressed as the mean ± SD. ^*P < 0.05, ^**P < 0.01 vs control group.