Research Paper Volume 12, Issue 12 pp 12305—12323

Inhibition of BMP3 increases the inflammatory response of fibroblast-like synoviocytes in rheumatoid arthritis

Figure 6. Overexpression of BMP3 inhibits the migration of RA and AIA FLS. (A) The protein levels of MMP-3, MMP-9, and TIMP-1 in TNF-α–treated RA FLS transfected with BMP3-pcDNA3.1 were analyzed using western blot. (B) The protein levels of MMP-3, MMP-9, and TIMP-1 in TNF-α–treated AIA FLS transfected with BMP3-PEX were analyzed using western blot. (C) The mRNA levels of MMP-3, MMP-9, and TIMP-1 in TNF-α–treated RA FLS transfected with BMP3-pcDNA3.1 were analyzed using qPCR. (D) The mRNA levels of MMP-3, MMP-9, and TIMP-1 in TNF-α–treated AIA FLS transfected with BMP3-PEX were analyzed using qPCR. (E) TNF-α–treated RA FLS were transfected with BMP3-pcDNA3.1, and their migration into the wound-healing site after 24 hours was photographed (original magnification, ×10). (F) TNF-α–treated AIA FLS were transfected with BMP3-PEX, and their migration into the wound-healing site after 24 hours was photographed (original magnification, ×10). All values are expressed as the mean ± SD. *P < 0.05, **P < 0.01 vs RA group. #P < 0.05, ##P < 0.01 vs NC-pcDNA3.1 group.